Our proposal's main objective is to realize cyber-physical platform and principles for (i) interrogating global modalities of intracellular transport with causative factors isolated at the single-molecule scale and (ii) realizing efficient and robust infrastructure for transporting micron/molecular scale cargo using distributed strategies We are realizing in-vitro, a transport network with roadways formed by microtubules where motorproteins, kinesin and dynein, will form vehicles ferrying cargo.